Senior Bacterial Core Lab Technician

Senior Bacterial Core Lab Technician

Requirements

• Bachelor’s degree in Biology, Microbiology, or a similar science field with 5 years of relevant laboratory experience.
• Minimum of 2-3 years performing BSL-3 laboratory operations and training in animal manipulations, including conducting animal studies.
• Minimum of 2-3 years performing in vitro MIC testing of potential therapeutic compounds against Tier 1 bacteria.
• Willingness to work with diversity sets of Bacillus anthracis, Yersinia pestis, Francisella tularensis, Burkholderia mallei, and Burkholderia pseudomallei.
• Minimum of 3-5 years conducting small animal studies, including preparation and administration of antibiotics and vaccines, and collection of animal body fluids and tissues.
• Minimum of 2-3 years working within IACUC approved protocols.
• Research experience in pharmacology, pharmacokinetics, and pharmacodynamics.
• Direct experience in in-process testing and characterization of biopharmaceutical products using mass spectrometry, chromatography, HPLC MS/MS.
• Minimum of 2-3 years experience with Applied Biosystems Analyst, Excel, and Mass Spec Quantitation Software MultiQuant.
• Minimum of 2-3 years performing animal necropsies, animal gavage, and animal blood collections.
• Minimum of 2-3 years performing equipment inventory management (GFEBS experience preferred).
• Minimum of 2-3 years performing laboratory management within BSL-2 and BSL-3 environments, including safety functions and chemical inventory.
• Must be able to obtain and maintain a NACI clearance.
• BPRP and CS-PRP enrollment required.

Responsibilities

• Responsible for BSL-3 laboratory operations and training in animal manipulations, performing approximately 5 animal studies in both DTRA funded projects and contracted work.
• Perform in vitro Minimum Inhibition Concentration (MIC) testing of potential therapeutic compounds against Tier 1 bacteria, testing over 72 compounds per year against 30-strain diversity sets.
• Participate in small animal studies, including preparation and administration of antibiotics and vaccines, and collection of animal body fluids and tissues.
• Conduct animal health exams to ensure compliance with IACUC approved protocols.
• Participate in weekly lab meetings, assist in development and design of in vitro and in vivo work, and present posters at scientific conferences.
• Support BSL-2 pharmacokinetics and toxicology studies, including drug and vaccine administration and collection of blood and tissues for analysis.
• Contribute to the in vivo pharmacology research program, determining pharmacokinetics and pharmacodynamics relationships and efficacy of optimized leads.
• Assist with data analyses and drafting reports for internal or external customers.
• Work with mass spectrometry, chromatography, HPLC MS/MS, and other biophysical techniques to support in-process testing and characterization of biopharmaceutical products.
• Perform bioanalytical data acquisition, analysis, and reporting using Applied Biosystems Analyst, Excel, and Mass Spec Quantitation Software MultiQuant.
• Complete approximately 25 media/reagent preparations per year.
• Assist with approximately 5 animal challenges and 10 bacterial challenge preparations per year.
• Perform approximately 400 animal injections and 400 animal observations per year.
• Support in vivo projects by completing approximately 500 bacterial burdens in animal tissue per year.
• Perform approximately 500 small animal necropsies per year.
• Process approximately 300 animal gavage and 125 animal blood collections per year.
• Place approximately 50 GFEBs orders per year for supplies and equipment.
• Maintain laboratories in BSL-2 and BSL-3.
• Perform or assist with BSAT Inventory Management weekly.
• Conduct or assist with Equipment Inventory Management twice a year in BSL-2 and BSL-3.
• Perform or assist with monthly Laboratory Management, including safety functions and chemical inventory.
• Review relevant literature monthly and assist in preparing standard operating procedures and manuscripts.
• Present research findings at one scientific conference per year, requiring CONUS or OCONUS travel.
• Maintain a GLP training folder and comply with all GLP regulations when applicable.
• Contribute to the design and oversight of 10 small animal studies, including drafting study plans and scheduling personnel.
• Contribute to study progress reports and scientific manuscripts, including statistical analysis, data compilation, figure preparation, and methods summary.
• Assist in establishing new material transfer agreements and cooperative research and development agreements with pharmaceutical companies and universities.
• Other duties as assigned by the supervisor.

Preferred Qualifications

• GFEBS experience preferred.