Research Technician – Cell Culture
| Company | PerkinElmer |
|---|---|
| Location | Gaithersburg, MD, USA |
| Salary | $47320 – $59280 |
| Type | Full-Time |
| Degrees | Bachelor’s |
| Experience Level | Entry Level/New Grad, Junior |
Requirements
- Bachelors Degree in science or chemistry
- Minimum 6 months of technical expertise in cell culture, general lab instrumentation, cell-based assays required.
Responsibilities
- Provide high quality laboratory support to the Cell Services team.
- Grow, maintain, and cryopreserve contamination-free mammalian cells (suspension & adherent).
- Preparation of cell pellets for histological processing.
- Provide daily, vials of tested, frozen cell lines to scientists.
- Isolate and cryopreserve primary PBMCs and further cell subsets from both whole blood and leukopaks.
- Isolate a variety of cell types from fresh blood daily in a timely manner.
- Isolate serum from whole blood.
- Maintain sterile technique while culturing isolated primary cells.
- Perform established flow cytometry QC protocols on a routine basis and analyze results.
- Generate and analyze data with the highest Data Integrity standards to include identifying and reporting unusual results/outcomes.
- Operate and maintain laboratory instrumentation including Mycoplasma onsite testing & clean-up as warranted.
- Continue technological improvements to increase productivity and cost-efficiency.
- Develop organization skills, presentation skills and attention to detail.
- Interact with senior staff members as necessary.
- Ensure compliance with all R&D data integrity practices, ethics codes, standards and training, and safety training and practice.
- May be requested to provide on-call service (primary and secondary) for specific research units in the lab areas.
Preferred Qualifications
- Previous experience in biotech and/or pharmaceutical drug discovery.
- Prior experience isolating primary cells preferred (Stemcell Technologies/Miltenyi)