Associate Scientist I-Cell and Gene Therapy

Associate Scientist I-Cell and Gene Therapy

Requirements

• BS required, MA/MS preferred in a related scientific discipline.
• 8 years of hands-on experience and in-depth knowledge of cell and gene therapy techniques and gene editing is required.
• Extensive experience in gene editing technologies, including CRISPR, base editing, prime editing, or transposase/recombinase-based editing.
• Strong background in molecular biology, with practical experience in NGS, molecular analytical development, gene editing design, and molecular cloning.
• Expertise in cell culture methodologies, development, and characterization of cell therapy products including HSCs, iPSCs, and/or CAR-T cells.
• Extensive experience in the molecular characterization of cell therapies, with a strong preference for expertise in NGS.
• Familiarity with CAR design, engineering, screening, and assay development is highly desirable.
• Experience in developing high-throughput screening platforms for cells, proteins, or antibodies is preferred.
• Background in antibody/protein engineering, protein production, and purification is highly preferred.
• Experience in virus production, transduction, and analytics is a valuable plus.
• Strong knowledge of industry standard gene editing techniques and analytics including CRISPR, guide RNA design and screens, indel analysis, knock-out and knock-in analysis, NGS, ddPCR.
• Solid understanding of cellular, molecular, and immunological techniques including cloning, ELISA, multi-color flow cytometry, in vitro functional assays, cell based assays, reporter assays.
• Experience in cell engineering processes including knowledge of cell line generation workflows and experience developing therapeutic engineered cells such as CAR-T.
• Expertise in cell culture techniques including culture of primary cells, iPSCs, HSCs, and cell lines from multiple species and cell manipulation techniques including transfection, nucleofection, and transduction.
• Experience with cell characterization including multicolor flow cytometry, FACS, MACS, and single cell isolation techniques.
• Experience in antibody or protein production, purification, and analysis is a plus.
• Proficient in MS Office Suite.

Responsibilities

• Support Cell and Gene Therapy pipeline projects by working with Project Leads to enable gene editing and generate new molecular workflows.
• Serve as a Project Lead for technology themed projects if needed.
• Identify, evaluate, and implement new gene editing technologies.
• Contribute scientific and technical knowledge to project and support teams.
• Design, initiate, and optimize molecular, cell based, and analytical methods to support the Research, Technical Operations, and In Vivo Pharmacology teams.
• Maintain compliance with regulatory guidelines and internal standards, and prepare necessary documentation for project reviews (proposals, monthly, quarterly, annual, or study reports), patent applications, publications, and/or animal, biosafety, or human subject protocols.
• Present research at project meetings and efficiently record experiments and data in laboratory notebooks, databases and monthly reports.

Preferred Qualifications

• Familiarity with CAR design, engineering, screening, and assay development is highly desirable.
• Experience in developing high-throughput screening platforms for cells, proteins, or antibodies is preferred.
• Background in antibody/protein engineering, protein production, and purification is highly preferred.
• Experience in virus production, transduction, and analytics is a valuable plus.