Molecular Virology Lead Technical Support

Molecular Virology Lead Technical Support

Requirements

• Master’s degree with at least five years of related laboratory post-degree experience in Biomedical Science, Microbiology, or Virology.
• Experience as a BSL-2, BSL-3, and BSL-4 lab supervisor and mentor.
• Experience supporting BSL-2, BSL-3, and BSL-4 laboratory inspections conducted by various regulatory bodies.
• Experience evaluating viral vaccines for immunogenicity using ELISA, flow cytometry, and other immunological techniques.
• Experience with molecular biology techniques including PCR, qRT-PCR, DNA preparations, cloning, FISH, flow cytometry, Western blot, IFAs, high content imaging, plaque assays, and PRNTs.
• Experience performing equipment inventory management (GFEBS and/or DMLSS preferred).
• Experience performing laboratory management within BSL-2, BSL-3, and BSL-4 environments, including safety functions and chemical inventory.
• Must be able to obtain and maintain a NACI clearance
• BPRP and CS-PRP enrollment required.

Responsibilities

• Serve as a mentor for BSL-3 and BSL-4 laboratories.
• Coordinate and collaborate on in vivo and in vitro studies to evaluate medical countermeasures, supporting approximately 10 small animal studies in BSL-2, BSL-3, and BSL-4 laboratories.
• Perform downstream analysis including viral replication assessment, transcriptomic analysis, blood chemistry, viral antigen and antibody detection, cytokine and chemokine levels determination.
• Use cell culture techniques to perform in vitro testing of candidate vaccines and therapeutic compounds, and maintain microphysiological systems.
• Plan, manage, and execute complex in vitro studies using organ tissue equivalents, organs-on-a-chip, and organoid systems.
• Design and perform molecular biology techniques including PCR, qRT-PCR, DNA preparations, cloning, FISH, flow cytometry, Western blot, IFAs, high content imaging, plaque assays, and PRNTs.
• Perform basic cell culture, oversee cryopreservation and thawing of cell lines, transfect cells, and produce viral stocks.
• Present protocols and data at weekly virology meetings.
• Maintain laboratory supplies in BSL-2, BSL-3, and BSL-4 laboratories, and order supplies using GFEBS and/or DMLSS ordering systems.
• Perform technical literature searches and assist in preparing proposals, technical reports, and manuscripts for publication.
• Present work at scientific meetings, requiring CONUS or OCONUS travel.
• Manage and safeguard BSAT, animal tissue samples, and working stock samples, ensuring current records are maintained.
• Assist with annual equipment inventory management in BSL-2, BSL-3, and BSL-4.
• Provide a monthly PI Technical Report.
• Perform other duties as assigned by the supervisor.

Preferred Qualifications

• Experience performing equipment inventory management (GFEBS and/or DMLSS preferred).